Test ABO blood group and Rh type

Test ABO blood group and Rh type. This video will guide you on how to test blood group at home with the help of Anti A, B and D (Rh) monoclonal antibody reagents.

Blood grouping and typing procedure:

• 1. Mix! First mix the each drop of blood with three different reagents including either of the three different antibodies, A, B or Rh antibodies!
• 2. Look for agglutination! Then you look at what has happened. ...
• 3. Figure out the ABO blood group! ...
• 4. Figure out the Rh blood group! ...
• 5. Figure out the blood type! Blood is often grouped according to the ABO blood typing system.

This method breaks blood types down into four categories: Type A Type B Type AB Type O Rh +ve or -ve Blood typing is also done to tell whether or not you have a substance called Rh factor on the surface of your red blood cells. If you have this substance, you are considered Rh+ (positive). Those without it are considered Rh- (negative). Rh typing uses a method similar to ABO typing. Normal Results ABO typing: If your blood cells stick together when mixed with: • Anti-A serum, you have type A blood • Anti-B serum, you have type B blood • Both anti-A and anti-B serums, you have type AB blood If your blood cells do not stick together when anti-A and anti-B are added, you have type O blood.

RH typing: • If your blood cells stick together when mixed with anti-Rh serum, you have type Rh-positive blood. • If your blood does not clot when mixed with anti-Rh serum, you have type Rh-negative blood. The last slide blood group is B -ve as you can see clumping/agglutination with Anti-B reagent and no agglutination with rest 2 Considerations

There are many antigens besides the major ones (A, B, and Rh). Many minor ones are not routinely detected during blood typing. If they are not detected, you may still have a reaction when receiving certain types of blood, even if the A, B, and Rh antigens are matched. A process called cross-matching followed by a Coombs' test can help detect these minor antigens and is routinely done prior to transfusions, except in emergency situations.